pone.0243333.s005.tif (9.46 MB)
Heatmap of Cq values from direct RT-qPCR experiments using various combinations of detergent on clinical samples.
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posted on 2021-04-14, 17:55 authored by Julien Fassy, Caroline Lacoux, Sylvie Leroy, Latifa Noussair, Sylvain Hubac, Aurélien Degoutte, Georges Vassaux, Vianney Leclercq, David Rouquié, Charles-Hugo Marquette, Martin Rottman, Patrick Touron, Antoinette Lemoine, Jean-Louis Herrmann, Pascal Barbry, Jean-Louis Nahon, Laure-Emmanuelle Zaragosi, Bernard MariSamples from a positive- or negative-COVID-19 patient collected in a commercial VTM was mixed with different combinations of detergents/emulsifiers, in presence or absence of PK (2 mg/mL) and further heat at 95°C for 5 min or not. Cq values obtained in quadruplicate are presented. Tx: Triton X100; PEG: poly ethylene glycol 600; QE: Quick ExtractTM DNA Extraction Solution.
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SARS-CoV -2 variantsoptimized processing bufferassaysetdetectioncapacityinactivating lysis buffersSARS-CoV -2 testing capacitiesRT-qPCRSARS-CoV -2sample PCR reaction volumes4608 qPCR end-pointshigh-throughput SARS-CoV -210 nL-range volumeRNA extraction stepmicrofluidic qPCR testBiomark TM instrumentBiomark TM reactionsresponseprimers
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