pone.0261853.g002.tif (207.89 kB)
Heat treatment inactivates SARS-CoV-2.
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posted on 2022-01-13, 18:44 authored by Margaret G. Mills, Emily Bruce, Meei-Li Huang, Jessica W. Crothers, Ollivier Hyrien, Christopher A. L. Oura, Lemar Blake, Arianne Brown Jordan, Susan Hester, Leah Wehmas, Bernard Mari, Pascal Barby, Caroline Lacoux, Julien Fassy, Pablo Vial, Cecilia Vial, Jose R. W. Martinez, Olusola Olalekan Oladipo, Bitrus Inuwa, Ismaila Shittu, Clement A. Meseko, Roger Chammas, Carlos Ferreira Santos, Thiago José Dionísio, Thais Francini Garbieri, Viviane Aparecida Parisi, Maria Cassia Mendes-Correa, Anderson V. de Paula, Camila M. Romano, Luiz Gustavo Bentim Góes, Paola Minoprio, Angelica C. Campos, Marielton P. Cunha, Ana Paula P. Vilela, Tonney Nyirenda, Rajhab Sawasawa Mkakosya, Adamson S. Muula, Rebekah E. Dumm, Rebecca M. Harris, Constance A. Mitchell, Syril Pettit, Jason Botten, Keith R. JeromeStocks of SARS-CoV-2 split into aliquots were treated at 95°C for 10 min or untreated. Viral titer was determined using a focus forming assay with an antibody recognizing the viral N protein. Measurements are in focus forming units (FFU) per milliliter (n = 4 replicates). The limit of detection for this assay was 20 FFU/ml.
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viral transport mediapurified nucleic acidsgenerate actionable datafewer experimental resourcesless &# 8221oral pharyngeal samplesconcordance across laboratoriesaccessible qualitative methodless &# 8220direct &# 8221pcr assay methods&# 8220participating laboratoriesnegative samplesdirect rtresults suggestreliable detectionqpcr methodpotently inhibitpcr reactionobserved 100lower costhouse equipmentfeasible optionequipment shortagesefficient covidcorrect identificationcontain charcoalcommon reagentscommon protocol2 rna2 detection2 ).
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