1471-2164-9-10-3.jpg (47.9 kB)
Halibut mitochondrial genomes contain extensive heteroplasmic tandem repeat arrays involved in DNA recombination-2
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posted on 2011-12-31, 13:41 authored by Kenneth A Mjelle, Bård O Karlsen, Tor E Jørgensen, Truls Moum, Steinar D JohansenCopyright information:
Taken from "Halibut mitochondrial genomes contain extensive heteroplasmic tandem repeat arrays involved in DNA recombination"
http://www.biomedcentral.com/1471-2164/9/10
BMC Genomics 2008;9():10-10.
Published online 11 Jan 2008
PMCID:PMC2248175.
FP) and a reverse primer (RP). Here, primer sets L16293/H17347 and L16376/H17369 were used on Atlantic/Pacific halibuts and Greenland halibut, respectively. () Separation of amplified products in a 2.5% agarose gel. M, the size marker 1 Kb Plus DNA Ladder from Invitrogen (right). Number of repeat motifs is indicated (left). The four smallest amplified fragments from Hh-2 (marked a, b, c, and d) were eluted from the gel and further sequence analysed. () Summary of sequence analyses of fragment from Hh-2. Note that fragment contains three repeats of the 61-bp motif (I*, II, II), fragment contains two repeat motifs (II, II), fragment contains a single motif (II), and fragment lacks a complete motif. Motif I* is not present in the plasmid cloned array of Hh-2 (Figure 2) and may represent site heteroplasmy at array motif position 33.