HNL1 favors larger substrates as compared to HbHNL.

The y-axis is the natural logarithm of the relative rate of the HNL1-catalyzed reaction versus the HbHNL catalyzed reaction, while the x-axis is the molecular weight of the substrate, which approximates its size. The open symbols indicate lyase-type reactions: open circles are cleavage of cyanohydrins and the open square is the cleavage of 2-nitro-1-phenyl ethanol (reverse of a nitro-aldol addition). The filled black circles indicate hydrolysis of esters. The best linear fit (dashed line) is y = 0.032±0.009·x– 5±1 and the r² = 0.61. Data for this figure are in S2 Table.