HIV from CSF escape contains the CD26 host surface marker consistent with T cell origin.

(A) Schematic of method. Cell-free virus was bound to columns with anti-CD26 antibodies linked to magnetic beads. After washing off unbound virus, virus bound to the columns was eluted and quantified using a viral load assay. (B) Monocyte derived macrophages or peripheral blood mononuclear cells were infected with NL4–3(AD8) macrophage tropic HIV and supernatants from infected cells were loaded on columns with CD26 antibody and normalized by viral input. Results were compared with virus derived from study participants with CSF escape. Shown are median and IQR of supernatants derived from 10 CSF escape participants or in vitro infections of PBMC or MDM from 7 healthy blood donors, where blood from one of the donors was used twice (n = 8 total experiments). p-values are * < 0.05; *** < 0.001 as determined by Kruskal-Wallis test with Dunn multiple comparison correction.