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pone.0266433.s002.tif (3.68 MB)

HEK293T ciliation data.

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posted on 2022-12-29, 18:30 authored by Arianna Ericka Gómez, Angela K. Christman, Julie Craft Van De Weghe, Malaney Finn, Dan Doherty

A) Proportion of ciliated HEK293T cells at 0, 24, 48, and 72 hours post serum starvation from additional thaws. B) Representative images of monolayer areas of HEK293T cell growth from Fig 1, Batch 3, which had the highest ciliation rates (top row) and repeat experiments with additional thaws of HEK293T cells. Cilia were stained with anti-ARL13B antibody. C) Representative images displaying ciliation in areas with multi-layer growth from additional thaws of HEK293T cells. Cilia were stained with anti-ARL13B antibody. Scale bars are 10μm.

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