HDAC11-S4 RNA 4 was validated by Northern blotting and in situ hybridization.

A, Validation of HDAC11-S4 RNA 4 by Northern blotting. The total RNA extracted from the BmCPV-infected midgut was separated on 1% agarose-formaldehyde gels (left) and transferred to Hybond-N+ nylon membranes. Northern blotting was conducted with biotin-labeled DNA targeting the junction site of chimeric HDAC11-S4 RNA 4 (Right). Total RNAs extracted from the non-BmCPV-infected midgut was used as a control. Lane M, DNA marker; Lane Con, total RNAs extracted from the non-BmCPV-infected midgut; Lane BmCPV, total RNAs extracted from the BmCPV-infected midgut. B, Validation of HDAC11-S4 RNA 4 by in situ hybridization. BmN cells with/without BmCPV infection at 48 h post-infection were digested with proteinase K and hybridized with biotin-labeled DNA targeting the junction site of chimeric HDAC11-S4 RNA 4. Hybridization signals were detected using CY3-labeled streptavidin. Cell nuclei were counterstained with DAPI.