pntd.0011878.g001.tif (376.34 kB)
Graphic presentation of alkDala (represented by ‘x’) labelling of intracellular bacteria in amoebal host [36].
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole CarntTrophozoites containing intracellular bacteria were exposed to AlkDala, facilitating its incorporation into the cell wall peptidoglycan, followed by a copper–catalysed click–chemistry reaction with an Azide–fluorophore (represented by green stars) to identify bacteria carrying the incorporated probe.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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