Gluconeogenic activity is maintained in Δfbp.sbp strains.

A–Changes in Δfbp.sbp cells grown in CMM and subjected to LC-MS metabolomics when compared to the parental cell line. The most changed metabolites are S7P, S1,7bP, and phosphothreonine. The plot includes 87 metabolites from glycolysis, PPP, TCA cycle or amino acid metabolism. B–Sedoheptulose 1,7-bisphosphate (S1,7bP), sedoheptulose 7-phosphate (S7P), fructose 1,6-bisphosphate (F1,6bP), and fructose 6-phosphate (F6P) as detected in the parental and Δfbp.sbp cells grown in CMM and subjected to LC-MS metabolomics. C—Δfbp.sbp cells were grown in HMI-11 supplemented with U-¹³C₃-glycerol and analysed by LC-MS metabolomics, showing that ¹³C from glycerol is incorporated into GNG products. H6P –hexose 6-phosphates. D—Δfbp.sbp cells were grown in CMM supplemented with glucose, or U-¹³C₃-glycerol and analysed by LC-MS metabolomics. G6P –glucose 6-phosphate.