pone.0260878.g001.tif (3.04 MB)

Glomeruli of PodoPalld129-/- mice have dilated capillaries and show a reduced expression of integrin α8.

figure

posted on 2021-12-08, 18:29 authored by Nadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole Endlich

(A) The periodic acid staining of paraffin kidney sections revealed dilated capillaries in 6 and 12 months old PodoPalld129-/- mice (asterisks). Scale bar represents 10 μm. (B) Quantitative analysis confirmed that PodoPalld129-/- mice of both age groups have significantly more glomeruli with dilated capillaries than controls of the same age. (C) Immunofluorescence staining of kidney sections showed a marked reduction of integrin α8 expression in PodoPalld129-/- glomeruli. Scale bar represents 20 μm. (D) Western blot quantification of Itga8 showed a significant decrease in 6 months old PodoPalld129-/- glomeruli (KO) compared to the control (WT; 6 months old PodoPalld129+/+ glomeruli protein lysates). Data are presented as means ± SD; * p<0.05; ** p<0.01; **** p<0.001; ns, not significant; two-way ANOVA with FDR correction (B) or unpaired Student’s t-test (D).