ppat.1010219.s010.tif (763.39 kB)
Global effects of itaconate and DI on transcriptomic responses in IAV-infected A549 cells.
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posted on 2022-01-13, 18:46 authored by Aaqib Sohail, Azeem A. Iqbal, Nishika Sahini, Fangfang Chen, Mohamed Tantawy, Syed F.H. Waqas, Moritz Winterhoff, Thomas Ebensen, Kristin Schultz, Robert Geffers, Klaus Schughart, Matthias Preusse, Mahmoud Shehata, Heike Bähre, Marina C. Pils, Carlos A. Guzman, Ahmed Mostafa, Stephan Pleschka, Christine Falk, Alessandro Michelucci, Frank PesslerA549 cells were infected with IAV (PR8M, MOI = 1) and gene expression was assessed by microarray analysis 24 h p.i. PCA based on the microarray analysis used for S11–S13 Figs, but additionally including treatment with 0.25 mM DI. The PCA shows normalization of IAV-driven reprogramming of gene expression, but increasing impact of itaconate on cellular responses with increasing doses. Toxicity data and dose-response curves of the compounds for HA mRNA and CXCL10 mRNA expression are shown in S2 and S9 Figs, respectively.
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