Germaria with reduced CENP-C exhibit a higher frequency of S-phase cells.

(A-D) 5 day old nanos-GAL4 and (A’-D’) CENP-C RNAi stained with DAPI (cyan), EdU (yellow) and 1B1 (red). * denotes cap cells. White dashed lines outline EdU positive GSC-CB (top) or cysts (bottom). Scale bar = 10 μm. (E) S phase index (%) of EdU positive GSCs in nanos-GAL4 and CENP-C RNAi (n = 150 germaria). (F) Violin plot showing the quantitation of EdU positive cysts per germarium (n = 150 germaria). One positive hit was quantified as a single EdU positive GSC/CB, 2-cell cysts (2cc), 4-cell cysts (4cc) or 8-cell cysts (8cc). ****p<0.0001.

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