ppat.1011435.s001.tif (1.31 MB)
Genotypic validation and additional phenotypes of the Af293 ΔhacA mutant.
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posted on 2023-10-31, 17:36 authored by Manali M. Kamath, Jorge D. Lightfoot, Emily M. Adams, Ryan M. Kiser, Becca L. Wells, Kevin K. Fuller(A) An overview of the hacA deletion strategy using in vitro assembled Cas9 RNPs confirmed by PCR of genomic DNA, (B) 72 h colony diameters of the indicated strains on YPD, GMM with ammonium tartrate as the nitrogen source (GMM-AT), GMM with BSA as the nitrogen source (GMM-BSA), or gelatin minimal medium. Data represented as mean of triplicate samples analyzed by two-way ANOVA **** <0.0001, (C) Colonial appearance of the indicated strains following growth on varying concentrations of brefeldin A (BFA) for 35°C for 48 h.
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unilateral blindness worldwideunfolded protein responseinfected lung samplesglucose minimal mediagelatin minimal mediafungus varies upondiv >< pdeletion mutant (<data altogether revealcomponent relay consistingbound irea proteinnutrient stress encounteredlevel stress ).high stress ),proper fungal adaptationimportant antifungal targettranscription factor hacaaspergillus fumigatus er stress experiencedacute er stressupr null mutantsfumigatus haca invasive pulmonary infectioninvasive pulmonarystress levelshaca important causefungal keratitiscorneal infectionunique hacahaca activityvitro upr contributestaken togetherresults indicateocular morbidityhost nichehost environmentsfurthermore avirulentemployed transcriptomicsdependent transcriptomebetter understand>&# 916
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