Genetic loss-of-function of Lipg and plasma triglycerides in mice.

A. Plasma TC, HDL-C, nonHDL-C, TGs, phospholipids, and nonesterified fatty acids from WT vs. Lipg^-/- mice after a 4 hour fast and again after 4 hours of refeeding a chow diet. Lipids were measured by chemical autoanalyzer. B. Percent change in each plasma lipid measure from (A) in 4 hour refed vs fasting measures. C. TG from plasma of fasted mice (left) and four hour-refed mice (right) fractionated by fast-protein liquid chromatography (FPLC) from pools from WT or Lipg^-/- mice from groups in (A). D. Total cholesterol, HDL-C, nonHDL-C, TGs, phospholipids, and nonesterified fatty acids in WT and Lipg^-/- mice after initiation of feeding adult mice a diet composed of 45% kilocalories fat for the indicated timepoints. Plasma was collected 4 hours after fasting and lipids were measured by autoanalyzer. E. Fasting plasma lipids after 7 weeks of high fat feeding were normalized to the levels at zero weeks of feeding and expressed as a percentage change. F. Triglycerides from fasted (left) and refed (mice) from (D-E) after FPLC-fractionation of plasma. A, B, E: *P<0.05, **P<0.01, ***P<0.001, Student’s unpaired T-test. D: **P<0.01, ***P<0.001, repeated factor 2-way ANOVA compared to WT group. Data is expressed as mean ± S.E.M.