Genetic characterization of the p{Ldh-GFP} transgene.

(A) A schematic diagram of the Ldh-GFP^Genomic transgene illustrating the placement of GFP immediately before the Ldh stop codon. When this transgene is placed in a Ldh^16/17 mutant background, the resulting Ldh-GFP fusion protein is capable of rescuing (B) the Ldh mutant lethal phenotype and partially restoring (C) lactate and (D) 2-hydroxyglutarate (2HG) accumulation. (E) The Ldh-GFP^Genomic temporal expression pattern is consistent with previous studies, with Ldh-GFP^Genomic being expressed at high levels throughout larval development and then declining at the onset of metamorphosis. Data in (B-D) analyzed using Kruskal–Wallis test followed by a Dunn’s multiple comparison test. ***P<0.001.