Generation of protein kinase C delta (PKCδ)-deficient mice.

(A) Schematic representation of murine Prkcd genes, targeting vectors, targeted allele, and knockout allele. Numbers denote the exon numbers, and light blue boxes denote 5ʹ- and 3ʹ- probes for Southern blotting. Polymerase chain reaction (PCR) primers for genotyping are indicated by arrows. Red letters E and B represent EcoRI and BamHI, respectively. Dashed line indicates vector sequence. (B) Southern blotting using genomic DNA of wild type (WT) and flox+/flox+ mouse. DNA digested using EcoRI was used for 5′-probes and shows sizes of 10.3 KB for WT and 8.3 kb for flox+/flox+ mice. DNA digested using BamHI was used for 3′-probes and shows sizes of 16.6 kb for WT and 15.4 kb for flox+/flox+ mice. (C) Genotypic analysis by PCR. PCR analysis was performed using genomic DNA extracted from 4-week-old mice tails and the amniotic membranes of fetuses. WT shows bands of 1078 bp, KO of 315 bp, and He of both 1078 and 315 bp.