ppat.1011978.s011.tif (922.12 kB)
Generation of SRPK2-knockout HepG2 cell line.
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posted on 2024-02-07, 18:40 authored by Ryan Pak Hong YIP, Doris Ching Ying Kwok, Louis Tung Faat Lai, Siu-Ming Ho, Ivan Chun Kit Wong, Chi-Ping Chan, Wilson Chun Yu Lau, Jacky Chi Ki NgoGenomic DNA from the cell lines was extracted for sequencing. The position where the DNA sequence of SRPK2 was altered by CRISPR-Cas9 is indicated (upper panels). Heterozygous sequencing results indicated that the knockout was biallelic and the altered DNA sequence in two alleles are labelled in red and blue respectively (bottom panels).
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pregenomic rna encapsidationelectron microscopy structureviral protein reactivatedviral nucleocapsid assemblyimportant cellular kinaseregulating capsid assemblyidentified cp sitesdiv >< pspecific docking grooveviral capsiddocking groovekinase utilizeshbv capsiddocking interactionvitro terminal domainpregenome packagingnew cryokinases decoratedirect interactionassays together>, whereas
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