pone.0260878.s001.tif (2.87 MB)
Generation of PodoPalld129-/- mice and confirmation of the podocyte-specific KO of palladin.
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posted on 2021-12-08, 18:29 authored by Nadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole Endlich(A) The specific palladin KO was confirmed by immunohistochemistry staining of paraffin kidney sections. PodoPalld129+/+ mice exhibit strong palladin-expressing podocytes (arrow). In contrast, there is no palladin signal (arrowhead) in PodoPalld129-/- podocytes. Scale bar represents 10 μm. (B) In addition, the palladin KO was verified by qRT-PCR (mean±SD, ***p<0.001; 6 months: Mann-Whitney U test, 12 months: unpaired Student’s t-test).
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parietal epithelial cells129 genetic backgroundmorphological deviations like12 months showedchronic kidney diseasesbinding protein palladin>, three palladinpodocyte foot processesdiv >< pbinding partners pdlim26 genetic backgroundproper actin cytoskeletonsignificantly reduced expressionproper podocyte morphologypodopalld129 -/- micepodopalld129 -/-podocyte morphologyreduced expressionmorphological changesimportant actinpodopalld129 -/-)kidney dependskidney damageintact morphologypodocyte spacetaken togetherspecific knockoutresults showproteinuria followedpdlim2 marked dilatationinteracting proteinsglomerular tuftfiltration barrierenlarged subclose contactback crossedage showed6 -/-)
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