ppat.1011983.s002.tif (478.32 kB)
Gating strategy for flow cytometry analysis.
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posted on 2024-01-25, 18:46 authored by Miguel A. B. Mercado, Qiang Li, Charles M. Quick, Yejin Kim, Rachel Palmer, Lu Huang, Lin-Xi LiRepresentative FACS plots depicting gating strategy for activated CD4 T cells in the FRT of infected WT and Bhlhe40-/- mice (as in Fig 2A). Dead cells and unwanted cells were excluded using a Dump channel containing antibodies against CD11b, F4/80, B220 and FVD (fixable viability dye).
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regulatory type 1protein basic helixfemale reproductive tractdiv >< pwhereas wt cd410r increased cd4key molecular driverheightened bacterial burdens>< sup ><decreased polyfunctional cd4>- deficient cd4exhibited severe defectschlamydia muridarum chlamydia polyfunctional responseskey regulator>mice deficientusing singletcr profilingsignificant enrichmentpartially rescuedmarked increaseintracellular bacteriumhost immunitygenetic ablationfunctional blockadedifferentiation trajectorycell signaturescell responsescell polyfunctionalitycell differentiationbacterial controlautoimmune diseases
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