ppat.1009402.s007.tif (2.27 MB)
Gating strategy for MLN and Peyer’s patches to identify monocytes and infected monocyte subsets.
figure
posted on 2021-03-11, 18:54 authored by Forrest C. Walker, Ebrahim Hassan, Stefan T. Peterson, Rachel Rodgers, Lawrence A. Schriefer, Cassandra E. Thompson, Yuhao Li, Gowri Kalugotla, Carla Blum-Johnston, Dylan Lawrence, Broc T. McCune, Vincent R. Graziano, Larissa Lushniak, Sanghyun Lee, Alexa N. Roth, Stephanie M. Karst, Timothy J. Nice, Jonathan J. Miner, Craig B. Wilen, Megan T. BaldridgeCells were gated as shown with a representative MLN sample from a Stat1-/- mouse infected with CW3. Cells were gated for live, single cells on the basis of FSC and SSC, followed by live/dead staining with LIVE/DEAD Aqua Stain. Inflammatory monocytes (Fig 7A) were identified as being CD11b+Ly6C+, and infected monocytes (Fig 7B) were identified as those inflammatory monocytes which were positive for MNoV NS1 or NS1/2.
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