Gastric CRF modulated hypothalamic CRF levels.
figureposted on 2018-09-07, 18:04 authored by Shin-ichiro Hagiwara, Esha Kaushal, Sreenivasan Paruthiyil, Pankaj J. Pasricha, Burcu Hasdemir, Aditi Bhargava
(A) CRF levels in the hypothalamus decreased after transient silencing of CRF in the stomach using RNAi in IA-treated rats (p<0.05; Student’s t-tests; IA dsCRF: 0.09 ± 0.05 vs. IA: 0.30 ± 0.06; n = 8/group) compared to control rats, reflecting a bidirectional feedback of the gut-brain axis. (B) Plasma CRF levels were not changed by either IA treatment or after transient silencing of CRF in the stomach. (C) Representative images of CRF-stained stomach sections of IA rats without (top row) and with (middle row) dsCRF treatment (scale bar = 50μm). Bottom row is negative control staining where primary antibody was excluded. Inset: Higher power (40x) images of the brush border of the mucosal layer and the myenteric neurons.
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rat modelCRF levelsCRF receptor 2CRF receptor 1plasma CRF levelsFGIDmodulating mast cell infiltrationdysregulated gut-brain interactionsRNATNFneonate rat pupsFDmast cell numbersGastric corticotropin-releasing factor influences mast cell infiltrationphosphorylated p 38degranulate mast cellsERKinfluence mast cell infiltrationMAPKIA-treated rats