ppat.1009476.s004.tif (1.52 MB)
Download file

GSK-LSD1 treatment downregulates Goblet cells in organoids.

Download (1.52 MB)
posted on 01.04.2021, 10:23 by Naveen Parmar, Kyle Burrows, Pia M. Vornewald, Håvard T. Lindholm, Rosalie T. Zwiggelaar, Alberto Díez-Sánchez, Mara Martín-Alonso, Madeleine Fosslie, Bruce A. Vallance, John Arne Dahl, Colby Zaph, Menno J. Oudhoff

(A) RT- qPCR for Muc2 expression in organoids left untreated or treated with GSK-LSD1 for 4 days. Expression was normalized to housekeeping gene Hprt. (B) Immunofluorescent images of MUC2 (green), UEA1 (red) and DAPI (Blue) in WT organoids that were cultured in normal culture medium (ENR = EGF, NOGGIN, R-SPONDIN) or in ENR medium supplemented with GSK-LSD1 for 4 days. Scale bar is 50 μm.