GSK-LSD1 treatment downregulates Goblet cells in organoids.
(A) RT- qPCR for Muc2 expression in organoids left untreated or treated with GSK-LSD1 for 4 days. Expression was normalized to housekeeping gene Hprt. (B) Immunofluorescent images of MUC2 (green), UEA1 (red) and DAPI (Blue) in WT organoids that were cultured in normal culture medium (ENR = EGF, NOGGIN, R-SPONDIN) or in ENR medium supplemented with GSK-LSD1 for 4 days. Scale bar is 50 μm.