GPR161 and SMO localization.

Representative images from each batch showing variation in A) SMO and B) GPR161 localization. C) Normalized fluorescence intensity (arbitrary units, au) of GPR161 with and without stimulation in an additional passage of hTERT RPE-1 cells. Black bars = median normalized fluorescence intensity. Small symbol = normalized fluorescence intensity of one cilium. Numbers of cilia measured are listed below each column in the graph. Representative cilia are shown next to the graphs for unstimulated (-SAG) and stimulated (+SAG) cells. Scale bars are 5 μm. T-test of equal variances was performed. p-values: * 0.01 to 0.05, ** 0.001 to 0.01, and *** <0.001.

(TIF)