Functional prediction of CR-specific DElncRNAs.

(A) qRT-PCR analysis to validate the RNA-seq results at three embryonic stages. CR-specific lncRNAs Ln-CR5 and Ln-CR6 were selected for validation. Data represent mean ± SEM (n = 3 independent experiments). (B) qRT-PCR analysis of CR-specific lncRNAs in primary neural cell culture assay after lentiviral transduction of control (H1) or Foxg1-knockdown. Data represent mean ± SEM (n = 4 independent experiments, **P<0.01, *P<0.05, T test). (C) In situ hybridization showing no expression in CR-specific lncRNA Ln-CR2 negative control (sense probe) at E11.5, E13.5 and E15.5, respectively. Scale bar, 900 μm. (D) Immunostaining of E15.5 Ebf2-EGFP+ brain sections shown nearly all (98.67%) Ebf2+ cells were Reln+ cells. RELN (red), GFP (green), DAPI (blue) for Reln+ CR neurons, Ebf2+ CR neurons, and nucleus, respectively. Scale bar, 50 μm.

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