Functional associations of valve cluster genes generated by unfiltered IPA analysis.

A) If no filtering is carried out, the HOTAIR and EIF2 signalling pathways are most strongly associated with the valve cluster data, whereas oxidative phosphorylation is negatively associated. B) Both HOTAIR and EIF2A are expressed in the valve endocardium (VEC) of developing aortic valves at CS19. HOTAIR is also expressed in VIC. The boxed area marks the area shown in high power (b,d). C,D) Cell movement/migration and proliferation-related terms are most strongly associated with the unfiltered valve dataset (compared with the myocardial), whereas terms relating to cell/tissue death and again congenital/familial heart/cardiovascular disease, including valvulopathy, (red box in C) were negatively associated. Scale bar in B = 200μm in a,c, 40μm in c,d.