Frequency and phenotype of DC and monocyte subpopulations in young vs old healthy donors compared to COVID-19 patients.

(A) Relative frequencies of DC subsets and non-DCs within the DC gate are shown. (B) Relative frequencies of classical monocytes (mo 1), intermediate monocytes (mo int) and non-classical monocytes (mo 2) within the monocyte gate are shown. (C) Relative frequencies of DC3 subtypes identified by CD163 and CD14 expression are shown (mean and SD). (D) Surface expression (MFI) of several markers shown in mo 1. (A-D) Healthy patients (= H1, black, n = 11), aged healthy patients (= H2, white, n = 6), mild/moderate COVID-19 pts (= M, red, n = 8) and severe COVID-19 patients (= S, blue, n = 2). Kruskal-Wallis Test with Dunn’s correction, or ANOVA and Tukey’s test was used, * p<0.05, ** p<0.01, *** p<0.001. (E) Frequencies of PD-L1^hi CD86^lo DC3 in patients receiving glucocorticoid therapy (white) and not receiving glucocorticoid therapy (black) are shown (n = 86). (F) Proportions of samples with more than 10% PD-L1hi CD86lo DC3 are shown as colored segments in the pie charts for patients with mild/moderate disease (M), severe disease (S), recovered patients (R) and controls (H, healthy donor and hospitalized non-CoV controls).

(TIF)