FoxO1 overexpression inhibited lipid accumulation and promoted lipolytic activity in palmitate-stimulated HepG2 hepatocytes.

Intracellular lipid accumulation was developed (A) by oil red O staining and (B) assessed by the optical density value of the collected oil red O dye. Original magnifying, 400×. Scale bars = 20 μm. (C) Intracellular lipid accumulation was measured by intracellular triglyceride assay. Lipolytic activity was determined by (D) the glycerol and (E) FFAs content in the cell media. Each bar represents the means ± SDs (n = 3). *P < 0.05, **P < 0.01.