Fixed granulocytes from SDS patients and healthy donors in flow chamber. Supplemented materials for article "Dissecting Thrombus-Directed Chemotaxis and Random Walk in Neutrophil Near-Thrombus Movement in Flow Chambers"

NET formation in flow chamber. Scale bar 10 μm. DNA – staining with Hoechst33342, NE – staining with antibodies against human neutrophil elastase, MPO – staining with antibody against human active myeloperoxidase.

Study approval statement: This study protocol for healthy donors was reviewed and approved by CTP PCP RAS Ethical Committee, approval number #1 from 12.01.2018. The study protocol for patients was reviewed and approved by the Ethical Committee of Pirogov Russian National Research Medical University (approval number #206 from 22.03.2021).