Figure S2 - Autoradiography and IHC control studies

Representative microscopy images of islets of Langerhans stained with (A, n=5) ¹²⁵I-GLP-1 amide, ¹²⁵I-exendin 4 amide and ¹²⁵I-exendin (9–39) amide autoradiography, (B, n=5) GLP-1R-antibody (brown DAB stain) derived from mice stimulated in vivo (20 min) with PBS, exendin-4 (GLP-1R agonist) or exendin (9-39) (GLP-1R antagonist) before sacrifice. The GLP-1R positive staining is located in both the membrane and the cytosol in mice treated with either PBS or exendin 9-39 (A and C) whereas there the GLP-1R positive staining is not located in the membrane in mice treated with exendin-4 (B). Counterstaining with hematoxylin. Bars=50µm.