Figure 3B

This data set contains the images that make up figure 3B. Images of biofilms grown with proteinase K, DNase or sodium metaperiodate followed by staining with fluorescent dyes of 55989 (AAF3) and K261 (AAF4) biofilms

Method: Biofilms were grown for 25 hours with media that contained 1 mg/ml proteinase K (prot K) 1 mg/ml DNase I (DNase), or 7.5 mM sodium metaperiodate (NaIO₄) or a vehicle control (PBS). After growth fixed biofilms were stained with Filmtracer Sypro Ruby Biofilm Matrix (red) for most classes of proteins, TOTO-1 iodide (green), an eDNA stain, wheat germ agglutinin (WGA) conjugate (violet) for glycoproteins, and Hoechst (blue) as a counterstain. Representative images from two experiments. Scale bar, 10µm

Paper doi: 10.3389/fcimb.2024.1379206

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