figshare
Browse

Figure 2. Gene expression analysis of DPPC populations by RT-PCR, at early PDs.

Download (455.91 kB)
Version 2 2016-04-14, 08:18
Version 1 2016-04-08, 14:31
figure
posted on 2016-04-14, 08:18 authored by Amr AlraiesAmr Alraies

Gene expression analysis of DPPC populations by RT-PCR, at early PDs.  All DPPCs showed positive expression for the MSC markers, CD73, CD90 and CD105; and the absence of the hematopoietic marker, CD45.  All DPPCs analysed were also negative for hTERT.  Only low proliferative capacity DPPCs (A1, A2, B1, C2 and C3) were positive for the expression of CD271 (nerve growth factor receptor p75, LNGFR) and for all 3 senescence-associated marker genes analysed, p53, p21waf1 and p16INK4a.  Replacement of cDNA with H2O served as negative controls, with β-actin serving as the reference housekeeping gene.

History

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC