Figure 2

LSFM of the anterior segment after ClearEye procedure.

From A to J, images are of anterior segments from six patients (age range 16-89 years). Panels A-D, F, G and I show anti-TubIII immunolabeling. Panel E and K shows ColIV labeling. Panel H shows anti-ColIV and TubIII labeling. Panels J show anti-ColIV and anti-SMA labeling.

A: Anterior segment section showing innervation of iris, cornea and ciliary bodies.

B: En face visualization of corneal innervation in a 16 year old donor.

C: Corneal innervation in an 89 year old donor with dry eye showing microneuromas (yellow arrowheads) and tortuous nerves (yellow square).

D: innervation of the ciliary bodies, iris and cornea.

E, F and G: scan of a collector vein (E, yellow arrowheads) downstream of the Schlemm’s canal (oval in E and F) with manual segmentation and 3D rendering (G) (see also supplementary video).

H: Double labeling of vessel (green) and nerves (magenta) in the anterior segment.

I: Innervation of iris.

J: Close up of the ciliary processes and scleral vascularization.

K: tracing of the pathway of a long anterior ciliary artery from outside the sclera (red), through it (magenta) to the ciliary muscles (blue) ending into the major arterial circle of the iris (green). (TubIII: Tubulin-III)