Figure 1
A: Graphic summary of ClearEye protocol.
B: Schematic representation of ocular tissues.
C: macroscopic aspect of a human ocular globe post-ClearEye procedure.
All subsequent panels (D to K) are from the same eye of an 84 year-old female. All panels show anti-CollIV immunolabeling except panel G and panel K, which show anti-alpha SMA labeling.
D: visualization of all vascular networks.
E: Sagittal section.
F: Pseudo-fundoscopic (en face) viewing of retinal vessels.
G: Low power en face view of choroidal vessels (draining vortex vein highlighted with yellow arrowheads) labeled using anti-alpha SMA.
H: Magnification showing the vessels from the optic nerve to the ora serrata squared in green in F.
I: magnification of perifoveal capillaries squared in yellow in F.
J optical section along the blue dotted line in panel I showing the microvascular layers of the retina (J)
K: optical section of the choroid (K). Note the high anti-alpha SMA labeling of choroidal arteries (See also supplementary video). (Col IV: collagen IV; αSMA: alpha-smooth muscle actin).