Fig 8 -

Production rates of sRNAs from different regions of virion (V) and complementary (C) stands of the viral genome in susceptible (S) and Ty-1 resistant (R) tomato plants infected with TYLCV-IL, its recombinant derivative TYLCV-IS76 or a combination thereof (IL+S76) at 10 (A) and 30 (B) days post inoculation (dpi). Illumina sRNA-seq reads in the size range from 20 to 25 nts representing virion and complementary strands of each transcription unit (V2-V1, C1-C4, C2-C3) and two parts of the intergenic region with the rightward (IR1) and the rightward (IR2) promoters of IL and IS76 genomes were counted in reads per million (RPM) of total (plant + viral) reads (see Material and Methods for further details of read counting in mixed infection). The resulting counts were divided by the length of each region in nucleotides and the load of respective viral DNA measured by qPCR and then multiplied by 10000. Bar graphs plot sRNA loads for the rightward (V2-V1) and leftward (C1-C4, C2-C3) mRNA transcription units and two parts of the intergenic region (IR1 and IR2). The loads of sRNAs derived from the virion and complementary stands of each region of the viral genome are represented with blue and red bars, respectively. In all cases, the loads are for two biological replicates per each condition, with the standard error shown with a capped vertical line and the mean value indicated above.