Fig 5 -

A: Cross-sections of the cardiac muscle were stained by the picrosirius red technique (PSR) and viewed with 40x objective and 400x magnification. B. Quantitative analysis of PSR-stained sections. C. PSR observed under polarized light with 40x objective and 400x magnification. The red and green colors are the collagens I and III, respectively. D. Quantitative analysis of collagen types. E. Histological sections of the cardiac muscle stained with Picrosirius-red staining after binarization at 40x objective and 400 magnification. F. Quantitative analysis of fractal dimension. Cont: controls groups. PAH: Pulmonary Arterial Hypertension groups; S: sedentary; T: training. S (n = 8): Sedentary Control; T (n = 8): Control with Training; SPAH (8): Sedentary Pulmonary Arterial Hypertension; TPAH (n = 8): Pulmonary Arterial Hypertension with Training. * p<0.05 compared to S One-way ANOVA and Tukey’s post hoc or Kruskal-Wallis and Dunn’s post hoc.