Fig 4 -

A) Regions of Nucleocapsid gene targeted by the diagnostic PCR-based tests. Primers are shown as continuous lines and probes–as dotted lines. Primers and the probe of the same test are shown in the same color. B) The open reading frames: Orf9b is shown in red, Orf14 –in blue and Nucleocapsid–in black. The regions coding for experimentally verified stable protein structures are shown as continuous lines and regions known to be structurally disordered—as dotted lines. Orf14, whose protein structure remains to be determined is shown as a dashed line. C) The rate of all (missense + synonymous) mutations per 100 nt windows. P-values from binomial tests are given for regions which are significantly under- or over-mutated (the entire Nucleocapsid ORF was used as a background).