Fig 2 -

Newly BRD4 recruitment and H3K27Ac translocation in vIl-6/THP-1 cells (A) Enrichment analysis by CHIP-Atlas. Enrichment analysis was performed on the list of genes whose expression were up-regulated by vIL-6 stimulation. The parameters used were as follows; cell type class: blood, threshold for significance: 100, threshold for log10 adjusted p value < -10. The Venn diagram shows the relatedness and number of genes up-regulated after vIL-6 stimulation in parental THP-1 (red circle) and vIL-6/THP-1 cells (green circle). (B) The predicted transcription factors or mediators selectively enriched only in vIL-6/THP-1 cells. The results were limited to THP-1 cells in CHIP-Atlas database. (C) CUT&RUN signals in ±5-kbp windows around the center of peaks in vIL-6/THP-1 cells. The list of peaks (p-value < 10−⁴) was extracted using findPeaks (HOMER with default parameters). Enrichment is shown in vIL-6/THP-1 (blue line) and in THP-1 (red line) cells. Images were drawn by plotProfile (HOMER). (D) H3K27Ac marks in parental THP-1 and vIL-6/THP-1 cells. The number of H3K27Ac peaks in vIL-6/THP-1 and parental THP-1 cells are depicted by a Venn diagram. (E) BRD4 CUT&RUN signals in vIL-6/THP-1 cells. The green line indicates BRD4 peaks±5-kbp around the center of H3K27Ac peaks in vIL-6/THP1 cells while the pink line indicates those in parental THP-1 cells. (F) Schematic model of H3K27Ac translocation and BRD4 recruitment at newly emerged H3K27Ac regions in vIL-6/THP-1 cells. (G) Distances of the nearest TSS to overlapping peaks of BRD4 binding and H3K27Ac enrichment sites. Total peak counts are shown. P values were calculated by the Kolmogorov–Smirnov test. (H) Annotation of overlapping peaks between BRD4 and H3K27Ac enrichment sites. Each annotation and its proportion were calculated by annotatePeaks.pl (Homer; parameters; default). Promoter regions are defined as ± 1-kbp from TSS. (I) BRD4 CUT&RUN signals in ±5-kbp windows around the center of CTCF peaks in vIL-6/THP-1 cells (blue) and parental THP-1 cells (pink).