pone.0258951.g002.tif (770.1 kB)
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posted on 2021-11-11, 18:35 authored by Dominic Leiser, Santanu Samanta, John Eley, Josh Strauss, Michael Creed, Tami Kingsbury, Paul N. Staats, Binny Bhandary, Minjie Chen, Tijana Dukic, Sanjit Roy, Javed Mahmood, Zeljko Vujaskovic, Hem D. Shukla(A) Knock out of CAV-1 gene by CRISPR CAS9 and effect of its overexpression in radioresistant A549 lung cancer cells. (A) Schematic diagram of generating radioresistant, CAV-1 KO and CAV-1 overexpressing A549 cells from parental A549 cells. (B), Survival curve of A549 radioresistant (Red) and CAV-1 KO cells (Blue), representing absorbed radiation dose and survival fraction using Linear-Quadratic Model. (C), Schematic diagram of generating CAV-1 knockout and CAV-1 overexpressing A549 cells by CRISPR-Cas9 gene editing technology. (D), Survival curve of A549 parental (Blue), and CAV-1 overexpressed cells (Red), representing absorbed radiation dose and survival fraction using Linear-Quadratic Model.
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