pone.0262160.g001.tif (1.23 MB)
Fe-specific chromatograms obtained after the analysis of plasma from MS participants, AIS participants and healthy controls.
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posted on 2022-01-12, 18:31 authored by Sophia Sarpong-Kumankomah, Katherine B. Knox, Michael E. Kelly, Gary Hunter, Bogdan Popescu, Helen Nichol, Karen Kopciuk, Henry Ntanda, Jürgen GailerColumn: Superdex 200 Increase 10/300 GL (30 x 1.0 cm I.D., 8 μm particle size), Temperature: 22°C, Mobile phase: 150 mM PBS buffer (pH 7.4), Flow rate: 0.75 mL/min, Injection volume: 500 μL. ICP-AES detection at 259.940 nm (Fe). Retention times of molecular weight markers are depicted on top. 750 μL of plasma was diluted with 250 μL of PBS buffer and 500 μL of the obtained solution were injected on the SEC column.
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