FD analysis of F-actin changes after treatment with Lat B using confocal or imitating widefield fluorescent microscopy.

Images of random cell from the control group were obtained by confocal (a1) or widefield fluorescent (b1) microscopy. Scale bar size is 100 μm, (a2) and (b2) show double-scaled images, and (a3) and (b3) show its’ skeletonized versions. (a4) and (b4) represent FD mean values and their standard deviations calculated for groups of control and Lat B-treated cells (n = 15 cells in each group) where F-actin images were obtained by confocal or imitating widefield fluorescent microscopy respectively. (*) comparing to DMSO-treated control group, p<0.05, each group is n = 15 cells.