Extended Data Figure 2

Extended Data Figure 2 | Glutamate evokes pericyte-mediated dilation of vasa recta capillaries. Data was taken from time series experiments in which naïve kidney slices were exposed to glutamate (glut; 10 μM) for approximately 500s. (a) shows a typical field of view for vasa recta before (ai), during (ii) and after (iii) exposure to 10 μM of glutamate. Pericytes are denoted by yellow dotted circle, red dotted lines show the pericyte site and the blue dotted lines show the non-pericyte site. b, Vasa recta capillary response to 10 μM glutamate. Black line shows pericyte site and grey line shows non-pericyte site. c, Mean constriction measured at pericyte site and non-pericyte site in response to different vasoconstrictors. Data shown from male Sprague-Dawley rats as mean ± s.e.m, n ≥ 3 pericytes. Statistics were calculated in GraphPad PRISM (5.0). Statistical significance between pericyte and non-pericyte sites were determined using: a Student's t-test for pericyte versus non-pericyte sites, *P < 0.05