Expression profiles and characterization of circMORC3.

(A) The expression levels of circMORC3 and MORC3 in liver samples after SCRV infection were detected by qRT-PCR. (B) mRNA levels of circMORC3 and linear MORC3 in MsbC cells measured by qRT-PCR after SCRV infection. (C) Confirmed the head-to-tail splicing of circMORC3 in the circMORC3 PCR product by Sanger sequencing. (D) PCR detection of circMORC3 and linear transcript of MORC3 by divergent and convergent primers in cDNA and gDNA of MsbC and MKC cells. (E) The stability of circMORC3 and MORC3 mRNA was detected by RT-PCR assay in the presence or absence of RNase R. (F) The stability of circMORC3 and MORC3 mRNA in MsbC and MKC cells was detected by qRT-PCR assay in the presence or absence of RNase R. (G) The existence of circMORC3 in MKC and MsbC was analyzed by nucleic acid electrophoresis and qPCR. All data presented as the means ± SE from at least three independent triplicated experiments. **, p < 0.01; *, p < 0.05 versus the controls.