Expression of TPI, VIM and MAT2A in sh₁ and sh₂.

Statistical analysis was performed using the Mann-Whitney U test for unpaired data. A. Normalised relative TPI expression of knockdown and control groups of HD1 and HD2. At each detection day, TPI expression was downregulated in sh₁ and sh₂. B. Normalised relative VIM expression of sh₁, SCR₁, sh₂ and SCR₂. VIM mRNA was significantly downregulated in sh₁ at day 15 and 25 and in sh₂ at day 25 and 50. Significant uprise in expression was observed in sh₁ at day 50. C. Normalised relative MAT2A expression of sh₁, SCR₁, sh₂ and SCR₂. MAT2A displayed a decrease in sh₁ and in sh₂ at day 5. Significantly higher expression was observed in sh₁ at day 15 and 50 and in sh₂ at day 50. D. Western blot analysis of TPI, VIM and MAT2A in knockdown and control groups of HD1 and HD2 at day 5, 15, 25 and 50. ACT was used as an internal control. *p<0.05, **p<0.01, ***p<0.001.