Expression of PDFR in l-LNvs regulates sleep in young flies.

(A) Sleep traces of Pdfr⁵⁵⁰⁴ mutants and Cs controls on day 0. (B) Sleep traces for Pdfr⁵⁵⁰⁴; c929-GAL4; UAS-Pdfr (rescue, green), Pdfr⁵⁵⁰⁴; UAS-Pdfr/+, Pdfr⁵⁵⁰⁴; c929-GAL4/+; Cry-Gal80/+, and Pdfr⁵⁵⁰⁴; c929-GAL4/UAS-Pdfr; Cry-Gal80 (n = 26–31/genotype). (C) Quantification of sleep during the WMZ of flies shown in (A). Cs flies sleep less during the WMZ than Pdfr⁵⁵⁰⁴ mutants (n = 26/genotype; t test, p < 0.05); (D) Quantification of sleep during the WMZ of flies shown in (B). Pdfr⁵⁵⁰⁴; c929-GAL4; UAS-Pdfr sleep less than parental controls; ANOVA F_[3,109] = 6.33, p = 5.4^E-4; n = 22–31. (E) Sleep is increased in Dcr2; c929-GAL4/UAS-Pdfr^RNAi flies on day 0 compared to Dcr2; c929-GAL4/+ and UAS-Pdfr/+ parental controls (ANOVA; F_[2,279] = 12.00, p = 1.04^E-5; n = 26–28). Data underlying this figure can be found in S2 Data. l-LNv, large ventral lateral neuron; PDFR, pigment dispersing factor receptor; WMZ, wake maintenance zone.