ppat.1009430.s003.tif (1.33 MB)
Expression of HLA-DR and CD86 on monocytes of Malian children and adults, and U.S. adults.
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posted on 2021-04-06, 17:24 authored by Rajan Guha, Anna Mathioudaki, Safiatou Doumbo, Didier Doumtabe, Jeff Skinner, Gunjan Arora, Shafiuddin Siddiqui, Shanping Li, Kassoum Kayentao, Aissata Ongoiba, Judith Zaugg, Boubacar Traore, Peter D. CromptonPBMCs from Malian children (aged 4–6 years; n = 9) and adults (n = 9) before the malaria season, as well as healthy malaria-naïve U.S. adults (n = 7) were gated for live monocytes and analyzed ex vivo by flow cytometry for surface expression of (A) HLA-DR and (B) CD86. t-SNE analysis of monocytes for all subjects in each group. Expression of each marker is indicated by a color scale.
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