pntd.0011878.g003.tif (451.5 kB)
Experimental flow chart of AK rat model.
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole CarntAfter a week of acclimatisation, intrastromal inoculation of Acanthamoeba (Ac) suspension (~2μl, ~104 trophozoites) was performed precisely using a 10μL micro–syringe with 33G needle. Slit lamp observation was performed from day 1 to 5 to examine the clinical features of keratitis progression. After infection period, eyes were harvested for pathological analysis of AK.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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