ppat.1009425.s005.tif (206.12 kB)
Exogenous rhamnolipids do not enhance PQS-dependent expression of pqsA in a tatA ΔpqsA mutant. PQS (40 μM) was added with or without purified rhamnolipids (50 μg/ml) to a pqsA mutant (grey bars) or a tatA ΔpqsA mutant (pink bars) carrying chromosomal pqsA’-lux fusions.
figure
posted on 2021-08-30, 17:38 authored by Eliza Ye-Chen Soh, Frances Smith, Maxime Rémi Gimenez, Liang Yang, Rebecca Munk Vejborg, Matthew Fletcher, Nigel Halliday, Sophie Bleves, Stephan Heeb, Miguel Cámara, Michael Givskov, Kim R. Hardie, Tim Tolker-Nielsen, Bérengère Ize, Paul WilliamsMaximal light output as a function of growth (RLU/OD600) is presented. Experiments were repeated in triplicate at least twice.
(TIF)
History
Usage metrics
Categories
Keywords
wild type levelsreduced bioluminescence revealedreduced antibiotic tolerancedependent quorum sensingdependent autoinduction resultingdiv >< pdefective biofilm architecture>< sub ><primary signalling defectidentify factors requiredpseudomonas aeruginosa tat prevented pqs>< sub xmlnsedna deficient biofilmsaeruginosa tat aeruginosa dependent qsp biofilm maturationtat tat substratesub xmlnsrieske sub>rhla pqse pqsa pqs phza1 lux cytc cytb 1 transposon librarysulfur subunitrieske ironqs ).parent strainmembrane vesiclemajor constituentgenetic complementationgenes resultsextracellular matrixextracellular dnaexogenous pqsenhanced susceptibilitydriven autoarginine translocationalso phenocopied34 validated
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC