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Exclusion of non-mycobacterial, DMN-tre+ organisms detected in bioaerosol samples based on cell morphology and staining profile.

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posted on 2021-02-01, 18:32 authored by Ryan Dinkele, Sophia Gessner, Andrea McKerry, Bryan Leonard, Ronnett Seldon, Anastasia S. Koch, Carl Morrow, Melitta Gqada, Mireille Kamariza, Carolyn R. Bertozzi, Brian Smith, Courtney McLoud, Andrew Kamholz, Wayne Bryden, Charles Call, Gilla Kaplan, Valerie Mizrahi, Robin Wood, Digby F. Warner

(A) Representative images of (i) C. striatum cultured in LB broth during log-phase and stained with 100 uM DMN-trehalose for 5 min, and (ii) DMN-tre+ organisms detected within a bioaerosol sample. (B) A panel of non-Mtb, DMN-tre+ organisms, as determined by our inclusion criteria, identified in various bioaerosol samples. Scale bar, 5 μm.

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