Excess of HMR interacts beyond the HMR core complex with chromatin architecture proteins (related to Fig 2).

(A) Volcano plot highlighting novel interactions gained by HMR upon overexpression. X-axis: log₂ fold-change of FLAG-HMR^endo IPs (right side of the plot) vs FLAG-HMR⁺ IPs (left side of the plot). Y-axis: significance of enrichment given as–log₁₀ p-value calculated with a linear model. HMR core complex subunits are labelled in red, novel factors enriched upon HMR overexpression are labelled in blue. Unlabeled additional bait-specific interactors are listed in S3 Table. (B) GO terms enriched upon overexpression of HMR. In (A) and (B) proteins were labelled or considered for GO search only if enriched in HMR⁺ or HMR^endo vs CTRL (p < 0.05) and differentially enriched between HMR⁺ and HMR^endo (log₂ fold-change (HMR^endo/HMR+) < 1.5).

(TIFF)