Example assay data from STB compounds.

(A) Motility assay example images. Tracked sperm cells treated with DMSO (top row), LRRK2-IN-1 (middle row) or Tafenonquine (bottom row). First (0 sec) and last frame (0.5 sec) of time-lapse movies are shown. Non-capacitating conditions (3h incubation with compound) are shown next to capacitating conditions (30 min incubation with compound). Compound concentration was 10 uM for all shown conditions. Colors in the non-capacitating assay indicate immotile sperm (pink), non-progressively motile sperm (lavender) or progressively motile sperm (teal). Colors in the capacitation assay indicate hyperactive sperm (green) or non-hyperactive sperm (blue). Scale bars 50 um. (B) Acrosome assay examples. Histogram depicting number of events and shift in intensity in the PNA acrosome channel (AR+ population). Acrosome assay protocol 1 (3h incubation with compound, no agonist addition; left panel) and acrosome assay protocol 2 (3h incubation with compound, 1h with agonist; right panel). Comparing DMSO (top row), LRRK2-IN-1 (middle row) and Tafenoquine (bottom row). Compound concentration was 10 mM for all shown conditions. Dashed line indicates gate (AR+ population).